Dr. Friederike Pfeiffer
Organization: Werner Reichardt Centre for Integrative Neuroscience
Phone number: +49 7071 29 89197
Position: Independent CIN Researcher
Scientific topic: Multiple Sclerosis
Field of Research
Multiple sclerosis is a disease which affects mostly young adults, resulting in increasing neurological constraints. During the course of the disease, mature oligodendrocytes are lost leaving demyelinated axons at a high risk to degenerate. Remyelination prevents axons from degeneration. Oligodendrocyte precursor cells are able to generate new myelin sheaths, a process which is possible but does not always occur in patients with multiple sclerosis especially at the later stages of the disease. The reasons for these differences and decreasing repair capacities remain unclear, mainly because the interactions between axons and oligodendroglial cells that promote remyelination are largely unknown. My current research focuses on investigating changes in the interactions that occur between demyelinated axons and oligodendroglial cells during the process of remyelination. Understanding these processes is crucial in order to develop new therapies that aim at enhancing remyelination.
This project is funded by the German Research Council (Deutsche Forschungsgemeinschaft, DFG).
Demyelination in the corpus callosum is induced by using Cuprizone and Lysolecithin models. The distribution of sodium channals isoforms and other proteins of interest during remyelination is studied by fluorescence techniques and confocal microscopy. The activity of nerve fibers is assessed through measuring compound action potentials. In addition, the ultrastructure is investigated by electron microscopy in cooperation with the electron microscopy department at the Institute of Pathology in Tübingen.
De- and Remyelination
Nodes of Ranvier
Pfeiffer F, Mack AF, Wolburg H: Topological aspects of the blood-brain and blood-cerebrospinal fluid barriers and their relevance in inflammation research; in ‘The Blood Brain Barrier and inflammation’ im Rahmen der Springer Serie: Progress in inflammation research, edited von Ruth Lyck and Gaby Enzmann, 2017, ISBN: 978-3-319-45512-9
Barzan R, Pfeiffer F, Kukley M (2016) N- and L-type voltage-gated calcium channels mediate fast calcium transients in axonal shafts of mouse peripheral nerve. Front. Cellular Neurosci. June, Volume 10, Article 135
Freeman SA, Desmazières A, Simonnet J, Gatta M, Pfeiffer F, Aigrot MS, Rappeneau Q, Guerreiro S, Michel PP, Yanagawa Y, Barbin G, Brophy PJ, Fricker D, Lubetzki C, Sol-Foulon N (2015) Acceleration of conduction velocity linked to clustering of nodal components precedes myelination. Proc Natl Acad Sci USA; Jan 20; 112(3): E321-8
Pfeiffer F, Schäfer J, Lyck R, Makrides V, Brunner S, Schaeren-Wiemers N, Deutsch U, Engelhardt B (2011) Claudin-1 induced sealing of blood-brain barrier tight junctions ameliorates chronic experimental autoimmune encephalomyelitis. Acta Neuropathol. Nov; 122 (5): 601-14
Döring A, Pfeiffer F, Meier M, Dehouck B, Tauber S, Deutsch U, Engelhardt B (2011) TET inducible expression of the 47-integrin ligand MAdCAM-1 on the blood-brain barrier does not influence the immunopathogenesis of experimental autoimmune encephalomyelitis. Eur J Immunol. Mar; 41 (3): 813-21
Boscacci RT, Pfeiffer F, Gollmer K, Checa Sevilla AI, Martin AM, Soriano SF, Natale D, Henrickson S, von Andrian UH, Fukui Y, Mellado M, Deutsch U, Engelhardt B, Stein JV (2010) Comprehensive analysis of lymph node stroma-expressed Ig superfamily members reveals redundant and non-redundant roles for ICAM-1, ICAM-2, and VCAM-1 in lymphocyte homing. Blood Aug 12; 116(6): 915-25
Wolburg-Buchholz K, Mack AF, Steiner E, Pfeiffer F, Engelhardt B, Wolburg H (2009) Loss of polarity marks blood-brain barrier impairment during experimental autoimmune encephalomyelitis. Acta Neuropathol. Aug; 118(2): 219-33
Pfeiffer F, Kumar V, Butz S, Vestweber D, Imhof BA, Stein JV, Engelhardt B (2008) Distinct molecular composition of blood and lymphatic vascular endothelial cell junctions establishes specific functional barriers within the peripheral lymph node. Eur J Immunol. Aug; 38(8): 2142-55, Cover Paper