Field of Research
Our main interest is to characterize functional properties of neural networks during development/processing of sensory information as well as mechanisms underlying their dysfunction in neurodegenerative diseases. Network development and the rules underlying processing of sensory information are currently studied in the olfactory bulb in collaboration with L.B. Cohen (Yale University) and M. Gˆtz (Helmholtz Center Munich). Network dysfunction in Alzheimer's disease is analysed in different mouse models of the disease. Here we are most interested in processes taking place in the vicinity of amyloid plaques (e.g. neuronal hyperactivity and silencing, rewiring of synaptic contacts, activation of microglia and astrocytes, etc.).
In vivo high resolution two-photon calcium imaging/electrophysiology in anestetized and awake mice, cell attached and whole-cell patch clamp recordings in situ (brain slices) and in vivo, analyses of cell function using genetically-encoded calcium indicators, immunohistochemistry, confocal microscopy
calcium signaling; degeneration / regeneration; developmental neurobiology; glia; in vivo imaging of cell function; molecular & cellular neurobiology; neuro-pathology; neuro-physiology; olfaction
- Busche MA, Eichhoff G, Adelsberger H, Abramowski D, Wiederhold KH, Haass C, Staufenbiel M, Konnerth A, Garaschuk O (2008). Clusters of hyperactive neurons near amyloid plaques in a mouse model of Alzheimerís disease. Science. 321(5896):1686-9.
- Heim N, Garaschuk O, Friedrich MW, Mank M, Milos RI, Kovalchuk Y, Konnerth A, Griesbeck O (2007). Improved calcium imaging in transgenic mice expressing a Troponin-C based FRET sensor. Nat Methods. 4(2):127-9.
- Garaschuk O, Milos RI, Konnerth A (2006). Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo. Nat Protoc. 1(1):380-6.