Dr. Maria Kukley
Organization: Werner Reichardt Centre for Integrative Neuroscience
Phone number: +49 (0)7071 29 89180
Department: CIN Neuron-Glia Interaction
Position: Head of Research Group
Area: CIN Members
Scientific topic: Neuron-Glia Interaction
Field of Research
We are interested in understanding the mechanisms by which neurons and glial cells interact to support normal communication in the nervous system. Directed action potential dependent fusion of neurotransmitter-filled vesicles occurs at synapses, points of contact between neurons, and enables rapid intercellular signalling in the brain. We have recently shown that action potentials also induce fast release of neurotransmitter onto glial cells in white matter - area of the brain that does not contain synapses. Ongoing projects in the lab include characterisation of this novel neuron-glia signalling during development, elucidating mechanisms and exploring functional role of neurotransmitter release onto glial cells in white matter. We are also investigating possible contribution to neurotransmitter release in white matter to the pathophysiology of demyelinating disorders of the nervous system.
Whole-Cell Patch-Clamp recordings of glial cells and neurons in brain slices, Electrical Stimulation of axons in brain slices, Flash-Photolysis of caged compounds, Immunohistochemistry, Confocal Laser Scanning Microscopy
brain imaging; degeneration / regeneration; developmental neurobiology; glia; molecular & cellular neurobiology; neuro-physiology
- Gallo V, Mangin JM, Kukley M, Dietrich D (2008). Synapses on NG2-expressing progenitors in the brain: multiple functions? J Physiol. 586(16):3767-81.
- Kukley M, Kiladze M, Tognatta R, Hans M, Swandulla D, Schramm J, Dietrich D (2008). Glial cells are born with synapses. FASEB J. 22(8):2957-69.
- Kukley M, Capetillo-Zarate E, Dietrich D (2007). Vesicular glutamate release from axons in white matter. Nat Neurosci. 10(3):311-20.